Welcome to the MBI website!

MBI offers specialized expertise in aquatic and terrestrial biological assessment, monitoring, and technical evaluation. Our goal is to assist our clients toward better management, restoration, protection, and improvement of our nation’s natural resources. MBI also offers specialized training in aquatic resource ecology, evaluation, and restoration, as well as personalized training to meet the unique needs of individuals and organizations.
Add us to your network!

MBI Releases New Primary Headwater Data

Author: Chris O. Yoder and Edward T. Rankin

Analysis of the Probabilities of the Classification of Small Headwater Streams as Primary Headwater Habitat (PHWH) and Warmwater Habitat (WWH) in Southwest Ohio

As part of a series of analyses of the attainability of aquatic life uses in small headwater streams in Ohio, MBI analyzed a regional dataset based on recent assessments in southwestern Ohio watersheds. Specifically this analysis focuses on small headwater streams that are at the “boundary” of watershed sizes between Primary Headwater Habitat (PHWH) and Warmwater Habitat (WWH) streams as each is defined and designated by Ohio EPA. Ohio EPA developed the PHWH methodology as a technique for evaluating and classifying the existing uses of small headwater streams that do not harbor fish assemblages capable of meeting the biocriteria for the WWH use designation as codified in the Ohio WQS (Ohio EPA 2002a). While there is no watershed size threshold that can accurately predict whether a stream will be capable of supporting either PHWH or WWH, “rules-of-thumb” have emerged and generally include a 1.0 mi.2 threshold for screening purposes. Some of these thresholds are being considered for routine use in regulatory applications such as 404 Nationwide Permits and without the benefit of vetting with actual site-specific monitoring data. One major problem is that biological and habitat data sufficient to determine the difference between PHWH and WWH (or any of the other Ohio aquatic life use tiers) is generally not collected in streams of this size thus leaving a significant knowledge gap to determine whether or not such rules-of-thumb are accurate or appropriate.

As part of intensive watershed monitoring conducted in the greater Hamilton County area of southwest Ohio, MBI routinely includes sufficient biological and habitat data collection in small headwater streams with some sites <0.5 mi.2 in drainage area. Furthermore, data was collected in a manner that allowed for a determination of whether a stream had the potential to attain one of the WWH aquatic life use tiers or if the existing use is more appropriately PHWH. This was performed at any stream site of <2.5 mi.2 based on the early Ohio EPA studies that resulted in the development of the PWHW classification scheme (Ohio EPA 2002b). Since 2011 a total of 96 stream sites of <0.5-2.5 mi.2 were sampled following this approach in the Mill Creek, Little Miami River, and Great Miami River study areas (Table 1). A fourth study area that includes similarly sized tributaries to the Ohio River mainstem were sampled in 2014, but those results have not yet been analyzed. The determination of the appropriate and attainable use designation was performed by Level 3 Qualified Data Collectors under project study plans approved by Ohio EPA. The techniques of data analysis and assignments of aquatic life use designations are the same as that practiced by Ohio EPA1 . Table 1 summarizes the results of this analysis.

1 Watershed assessment reports are available at: http://www.msdgc.org/initiatives/water_quality/index.html

The results are reported in 0.5 mi.2 increments of drainage area and by each of the three major watershed areas and as a total across all areas. The number of sites in each drainage area panel, the number that were assigned the WWH use designation, and the number that were assigned to the PHWH existing use classification are depicted in Table 1. The PHWH existing use assignments are further stratified as PHWH Class 1, 2, and 3. The attainment status of the WWH designated sites are indicated as full/partial/non-attainment in terms of observed attainment of the attendant numeric biocriteria for fish and macroinvertebrates.

Table 1. Results of the analysis of 96 small headwater stream sites of <0.5-2.5 mi.2 in southwest Ohio for the Primary Headwater Habitat (PHWH) existing use classification or the Warmwater Habitat (WWH) use designation.

Overall, 53 of the 96 sites (55.2%) were designated as having WWH potential either because the biological results indicated attainment of the WWH biocriteria or the habitat assessment indicated WWH potential even though the biological data indicated partial or non-attainment. The remaining 43 sites were classified as PHWH existing use Class 1, 2, or 3. More than one-half (53.5%) of these sites were classified as PHWH 3A which means that aquatic salamanders and sufficient macroinvertebrate taxa were observed. Seventeen (17) sites were classified as PHWH 2 (lacking salamanders) and only 3 sites as PHWH Class I, which is due to ephemeral flows, but which retain sufficient channel characteristics. None of the 96 sites were considered to warrant designation as Limited Resource Waters (LRW), although this use designation applies to some larger and highly modified stream segments in the Mill Creek and Little Miami study areas. At least one formerly designated LRW headwater stream segment was changed to PHWH as a result of the focused watershed assessments. In this case, the LRW designation was based on an upstream extrapolation from a single sampling site located downstream. This example illustrates the importance of having sufficient data collection and spatial resolution in the sampling design. PHWH existing use classifications were more frequent in the smaller drainage area panels and were included in all of the drainage area panels. WWH was assigned in all of the panels including those <0.5 mi.2 and <1.0 mi.2.

These results demonstrate that applying a simple “rule-of-thumb” such as 1.0 mi.2 as the size limitation for the WWH designation would have resulted in the erroneous classification and/or designation of 22 of 47 sites draining <1.0 mi.2 (46.8%). It also illustrates that simply extrapolating uses to apply to the upstream reaches of a stream has the potential for similar error propagation. Sufficient data collection and spatial scale resolution are both needed to avoid making errors in applying policies and permitting in small headwater streams. This means that for streams with permanent water and especially those with flow should require data collection that meets the provisions of the Ohio WQS and Ohio EPA methods. In such cases this will need to include the collection of fish, macroinvertebrates, and salamanders along with the QHEI as the principal habitat assessment (HHEI can be extracted from a QHEI). This promotes sound data as driving the decisions about designated and existing uses in small headwater streams. These results should be applicable beyond southwestern Ohio to similar topographies in the rest of Ohio.


Ohio EPA. 2002a. Field Evaluation Manual for Ohio’s Primary Headwater Habitat Streams. Final Version 1.0. Division of Surface Water, Columbus, OH. 60 pp.

Ohio EPA. 2002b. Ohio EPA Primary Headwater Habitat Initiative Data Compendium, 1999-2000 Habitat, Chemistry, and Stream Morphology Data. Division of Surface Water, Columbus, OH. 23 pp. + appendices.

Squaw Root

Author: Pete Precario

One of the most interesting plants I recently stumbled upon – literally – in northeastern Ohio is the American Squawroot (Conopholis americana). It goes by a number of common names including cancer-root and bear corn. It is an achlorophyllous plant, a big word that simply means that it doesn’t have any chlorophyll. Consequently it is not green and doesn’t photosynthesize any of its own food. Individual plants are from 4 to 8 inches tall and often well camouflaged by leaf litter.

Because it doesn’t produce its own food supply, Squawroot is a true parasitic plant. It is parasitic exclusively with oak trees and feeds off of their roots. At the point where the Squawroot attaches to the oak root a nodule or lump often forms leading to one of its common names -“cancer-root”. Unlike a lot of parasites, it does not do any apparent damage to the oak tree host and appears to live in harmony and balance with its food supply.

Because it blooms early in the spring, it is also reputed to be a favorite food of bears just coming out of hibernation. This is the apparent source of another of its common names – “bear corn”. Squawroot apparently does have some astringent medicinal properties. Reputedly it has also been used in tea or concentrated form for relief of headaches and pain.

The genome of the Squawroot has been sequenced. Interestingly, the genes related to photosynthesis are missing while the rest of the genome is largely similar and consistent with other vascular, seed-bearing flowering plants. This leads some to the conclusion that there was a genetic mutation responsible for the current dramatic nature of the plant.

While there are no flowers apparent on the pictured plants, Squawroot is a flowering plant. It produces seeds that work their way into the ground looking for oak roots rather than growing toward the sunlight. If you’re lucky enough to encounter the Squaw root stop and take a close look. It is a remarkable plant.

Spotted Salamander

The Midwest Biodiversity Institute Announces 2015 Training Courses

MBI offers a wide range of high quality courses for anyone interested in increasing their knowledge and skills in aquatic resource ecology. MBI's educational offerings cover many aspects of wetland and aquatic resource monitoring and bioassessment, including courses that provide certification as Level 2 and Level 3 Qualified Data Collectors under the Ohio Credible Data Law. We also offer personalized training tailored to the needs of your agency or organization.

MBI is offering the following courses for the 2015 season:
  • Wetland Delineation: April 27 – April 30 (Cost: $975.00 early/$1,025.00 standard)
  • Qualitative Habitat Evaluation Index (QHEI): May 5–6 June 1–2 (Cost: $475.00 early/$525.00 standard)
  • Primary Headwater Habitat (PHWH): May 7 – 8 (Cost: $475.00 early/$525.00 standard)*If taking both the May 5-6 QHEI and the May 7-8 PHWH, receive a discount.*
  • Amphibian IBI/ID: May 20 – 21 (Cost $475.00 early/$525.00 standard)
  • Ohio Rapid Assessment Method (ORAM) for Wetlands: May 27 – 28 (Cost: $475.00 early/$525.00 standard)
  • Ohio Credible Data Program Training - fish or macroinvertebrate specialties: June 1 – 5 (prices vary – check website for details)
  • Vegetation Index of Biotic Integrity (VIBI): June 16 – 18 (Cost: $725.00 early/$775.00 standard)
  • Identification of Common Wetland Plants: June 23 – 25 (Cost: $725.00 early/$775.00 standard)
  • Wetland Restoration: July 14 –15 (Cost: $525.00 early/$575.00 standard)

  • We also provided a variety of personalized training to multiple organizations.

    For more information about MBI and its training program, or to register for courses: Visit our education website by clicking the training link in the menu.

    2015 Vernal Pool Follow-Up Expedition

    Saturday, June 13, 2015 at 9:00AM

    Vernal Pool Expedition:

    Gahanna Woods State Nature Preserve

    Ohio Vernal Pool Partnership (Midwest Biodiversity Institute and Ohio Wetlands Association)

    In late winter to early spring, vernal pools come alive with the mating calls and breeding activities of adult amphibians. Those who attended our early spring workshop discovered that adult amphibians are only in the pools for a short period and then leave. Join amphibian biologist, Mick Micacchion in an expedition to the vernal pools at Gahanna Woods State Nature Preserve. Learn what has happened in the pools since amphibian eggs were laid in early spring. There will be a brief presentation on life in the pools during the spring months, and we will then visit vernal pools where all will have an opportunity to see larval and young adult amphibians.

    Meet at the parking lot on Taylor Station Road. The expedition involves a round trip hike of about 1 mile over easy to travel trials, hiking boots are recommended. All are welcome!